Sample requirements
MAF accepts DNA extracted from most materials, e.g. blood, cells, saliva, whole genome amplified (WGA) and filter paper spot is accepted on a case by case basis.
MAF recommends using a fluorometric technology such as PicoGreen® or Qubit® to measure DNA concentration. For Agena methods, spectrophotometric DNA concentration measurements are also accepted.
Any DNA left following the completion of a project, can be returned on request.
| Agena genotyping | Agena methylation | Illumina genotyping | Illumina methylation | |
| Concentration: | 4 ng/µL | 11 ng/µL | 11 ng/µL | 11 ng/µL |
| Volume: | >15 µl | >50 µl | >50 µl | >100 µl |
| Plate type: | ABgene AB-0800 | ABgene AB-0800 | ABgene AB-0800 | ABgene AB-0800 |
| Number of Empty wells/96 well plate: | 6 | 4 | 3 | 3 |
Sending samples to MAF
Plates should be labelled as in the corresponding Plate_layout file. Prior to sending samples to MAF: download the Delivery Note form, fill it in and print it out. This Delivery Note printout should then accompany the samples to MAF.
If sending samples via aircraft, seal plates with tight cap strips or send the DNA dried in the plates. Plates sealed with PCR film may leak their contents when shipped via aircraft.
Sample delivery address:
Mutation Analysis Facility, MAF
KFC, Novum plan 5
Hälsovägen 7
141 57 Huddinge
SWEDEN
Contact person – Agena projects:
Ann-Charlotte Rönn, PhD (Project coordinator)
Tel: +46 8 5858 3662
email: ann-charlotte.ronn@ki.se
Contact person – Illumina projects:
Astrid Fungmark (Laboratory engineer)
Tel: +46 8 5858 3642
email: astrid.fungmark@ki.se